[TQ1100] ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX), 200 RXN

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      Description 

      The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system. 


      Features

      • High sensitivity and signal intensity

      • Smart blue contrast dye as a visual aid for reaction setup

      • Better compatibility for reverse transcription

      • Low background 


      Storage 

      Protected from light.
      Aliquot to avoid multiple freeze-thaw cycles.

      -20°C for 12 months


      Odoo - Sample 1 for three columns

      Sensitivity

      The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.

      Odoo - Sample 2 for three columns

      Better Compatibility for reverse transcription 

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.


      Odoo - Sample 3 for three columns

      Specificity 

      The standard curve (left) and melting curve (right)  of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).



      Contents


      Component

      Volume 

            Cat. No

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      1 ml x 2

            TQ1100 (200 Rxn)

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      1 ml x 5

            TQ1101 (500 Rxn)


      Storage 

      Protected from light. 
      Aliquot to avoid multiple freeze-thaw cycles.

      -20°C for 12 months

       



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      Vet Med Sci. 2024 Nov;10(6):e70056. doi: 10.1002/vms3.70056.

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      Hermansyah D, Syarifah S, Muhar AM, Putra A.

      Breast Cancer (Dove Med Press). 2024 Jul 2;16:319-328. doi: 10.2147/BCTT.S457548. eCollection 2024.

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      Downregulation of salivary miR-3928 as a potential biomarker in patients with oral squamous cell carcinoma and oral lichen planus.

      Farshbaf A, Mohajertehran F, Aghaee-Bakhtiari SH, Ayatollahi H, Douzandeh K, Pakfetrat A, Mohtasham N.

      Clin Exp Dent Res. 2024 Apr;10(2):e877. doi: 10.1002/cre2.877.

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      Evaluation of the Antiviral Activity of Tabamide A and Its Structural Derivatives against Influenza Virus

      Soo Yong Shin, Joo Hee Lee, Jin Woo Kim, Wonkyun Ronny Im, Kongara Damodar, Hyung Ryeol Woo, Won-Keun Kim, Jeong Tae Lee, Sung Ho Jeon

      Int J Mol Sci. 2023 Dec 9;24(24):17296. doi: 10.3390/ijms242417296.

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      Exogenously applied Casuarina equisetifolia leaf extracts act as an osmoprotectant on proline accumulation under drought stress in local rice from Indonesia.

      Salsinha YCF, Rini DS, Indradewa D, Rachmawati D, Alam T, Purwestri YA.

      Front Plant Sci. 2023 Aug 3;14:1210241. doi: 10.3389/fpls.2023.1210241. eCollection 2023.

      PMCID: PMC10437820


      Isolation and characterization of the lactobacillus strain from honey and its probiotic properties.

      Goli Mehdi Abadi ME, Hosseini-Safa A, Habibi S, Dehghan M, Forouzani-Moghaddam MJ, Oshaghi M.

      Iran J Microbiol. 2023 Jun;15(3):439-447. doi: 10.18502/ijm.v15i3.12905.

      PMCID: PMC10336281


      Thymosin alpha 1 restores the immune homeostasis in lymphocytes during Post-Acute sequelae of SARS-CoV-2 infection.

      Minutolo A, Petrone V, Fanelli M, Maracchioni C, Giudice M, Teti E, Coppola L, Sorace C, Iannetta M, Tony Miele M, Bernardini S, Mastino A, Sinibaldi Vallebona P, Balestrieri E, Andreoni M, Sarmati L, Grelli S, Garaci E, Matteucci C.

      Int Immunopharmacol. 2023 May;118:110055. doi: 10.1016/j.intimp.2023.110055. Epub 2023 Mar 22.

      PMCID: PMC10030336 


      LY6K depletion modulates TGF-beta and EGF signaling.

      Park S, Park D, Han S, Chung GE, Soh S, Ka HI, Joo HJ, Yang Y.

      Cancer Med. 2023 Jun;12(11):12593-12607. doi: 10.1002/cam4.5940. Epub 2023 Apr 19.

      PMCID: PMC10278532


      Evaluation the effect of ZnO nanoparticle derived Bacillus subtilis on the expression of efflux pump genes (AdeB AdeRS) in Acinetobacter baumannii.

      Saleh F, Kheirandish F, Hosseini F, Yazdian F.

      J Environ Health Sci Eng. 2021 May 26;19(1):1133-1141. doi: 10.1007/s40201-021-00679-w. eCollection 2021 Jun.

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      The effect of dietary supplementation of coenzyme Q10 on reproductive variables of cadmium-challenged male Japanese quails (Coturnix Japonica).

      Irani S, Zhandi M, Sadeghi M, Yousefi AR, Marzban H, Rafieian-Naeini HR.

      Vet Med Sci. 2023 Mar;9(2):837-850. doi: 10.1002/vms3.990. Epub 2022 Nov 1.

      PMCID: PMC10029911


      Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

      Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

      Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328


      Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

      Mohamad Javad Forouzani‐Moghaddam, Sina Habibi, Ahmad Hosseini‐Safa, Khadijeh Khanaliha, Roya Mokarinejad, Fatemeh Akhoundzadeh, Mojgan Oshaghi

      Vet Med Sci. 2024 May; 10(3): e1407. Published online 2024 Mar 22. doi: 10.1002/vms3.1407

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      Adiponectin affects the migration ability of bone marrow-derived mesenchymal stem cells via the regulation of hypoxia inducible factor 1α

      Sujung Soh, Sora Han, Hye In Ka, Se Hwan Mun, Woojung Kim, Gaeun Oh, Young Yang.   

      Cell Commun Signal. 2023; 21: 158. Published online 2023 Jun 27. doi: 10.1186/s12964-023-01143-y

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      Nucleic Acids Res. 2022 Oct 14; 50(18): 10756–10771. Published online 2022 Sep 27. doi: 10.1093/nar/gkac799

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      The issue beyond resistance: Methicillin-resistant Staphylococcus epidermidis biofilm formation is induced by subinhibitory concentrations of cloxacillin, cefazolin, and clindamycin 

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      PLoS One. 2022; 17(11): e0277287. Published online 2022 Nov 9. doi: 10.1371/ journal.pone.0277287

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      Integrative Metabolomic and Lipidomic Profiling of Lung Squamous Cell Carcinoma for Characterization of Metabolites and Intact Lipid Species Related to the Metastatic Potential

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      Effects of coenzyme Q10 on reproductive performance of laying Japanese quail (Coturnix japonica) under cadmium challenge

      Heayyean Lee, Hwanhui Lee, Sujeong Park, Myeongsun Kim, Ji Young Park, Hanyong Jin, Kyungsoo Oh, Jeehyeon Bae, Young Yang, Hyung-Kyoon Choi, Cancers (Basel). 2021 Aug 19;13(16):4179. doi: 10.3390/cancers13164179.

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      Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

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      Hydroxylation and sulfation of sex steroid hormones in inflammatory liver 

      Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

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       ExcelTaq™ 2X Q-PCR Master Mix

      Product Name

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

      ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

      ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

      ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

      Cat. No.

      TQ1100

      TQ1110

      TQ1200

      TQ1210

      TQ2110

      Detection chemistry

      SYBR

      SYBR

      SYBR

      SYBR

      TaqMan

      Blue contrast dye for best convenience

      ü

      ü

      ü

      ü

      X

      ROX dye for reference

      X

      ü

      X

      ü

      ü

      Time required for enzyme activation

      10 min

      10 min

      2 min

      2 min

      10 min

      qPCR program

      Standard

      Standard

      Fast and Standard

      Fast and Standard

      Standard