[TP2000] ExcelTaq™ Blood Direct DNA Polymerase, 5 U/μl, 500 U

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Description 

The ExcelTaq™ Blood Direct DNA Polymerase is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct DNA Polymerase is highly tolerant in the presence of PCR interfering/ inhibiting substances in blood, such as IgG, hemoglobin, and lactoferrin. ExcelTaq™ Blood Direct DNA Polymerase is compatible with most anticoagulants, such as citrate, EDTA, and heparin (Fig. 1). The ExcelTaq™ Blood Direct DNA Polymerase includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.


Features

  • 5'→3' DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • Compatible with most anticoagulants 


Applications 

  • Direct amplification of DNA from blood samples

  • High throughput screening without DNA purification

  • Suitable for multiplex PCR


Storage

-20°C for 24 months

   4℃ for 6 months

Odoo - Sample 1 for three columns

Compatible with most anticoagulants 

ExcelTaq™ Blood Direct DNA Polymerase amplified 200 bp from differently treated blood using CCR5 specific primers. (M: DM2100)

 Contents

Component

Volume    

Blood Direct DNA Polymerase (5 U/μl)

100 μl      

5X Blood Direct Buffer

1 ml x 4

Positive Control Primers (10 μM, each)

50 μl      


Storage Buffer

20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol


CCR5 control primer sequence

CCR5-F: 5'-CTCCCAGGAATCATCTTTACC-3'

CCR5-R: 5'-TCATTTCGACACCGAAGCAG-3'


Storage

-20°C for 24 months 

 

Recommended PCR Condition

Blood

3 μl

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

5X Blood Direct Buffer

10 μl

dNTPs

0.2 mM (each)

Blood Direct DNA Polymerase

0.25 μl (1.25 U)

ddH2O

to 50 μl

Total volume

50 µl

Note: Mix well before PCR Reaction

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

3 min

1

Denature

94°C

30 sec

30-40

Annealing

50-68°C*

30 sec

Extension

72°C

60 sec/kb

Final extension

72°C

3 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.

 

Post amplification analysis

Centrifuge PCR reaction mixture at 1000 × g for 1 ~ 3 minutes. Load only the clear supernatant of the PCR products for gel electrophoresis analysis.

Odoo - Sample 3 for three columns

[TP2100] ExcelTaq™ 5X Blood Direct PCR Master Mix Kit

  • 5'→3' DNA polymerase activity

  • None detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High throughput PCR

  • Execute PCR directly from blood samples 

  • High reproducibility, less pipetting errors

  • Compatible with most anticoagulants 

Odoo - Sample 1 for three columns

[TP5000] ExcelTaq™ Hot Start II DNA Polymerase

  • Aptamer-based hot start PCR

  • Reversible enzyme inactivation

  • Omits extra enzyme activation step

  • Convenient for room temperature PCR set-up

  • High yield and specificity of target amplicons

  • Wide range of amplicon length (up to 10 kb)

  • High sensitivity (as low as 1 fg of plasmid)

Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High Stability

  • Fast Hot Start

  • High Sensitivity

  • Low Background / High Specificity 

  • Suitable for Fast Program 

  • Smart Blue Contrast Dye 

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR