[RP1300] ExcelRT™ Reverse Transcription Kit, 100 RXN

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ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 


  • Contains all components for reverse transcription 

  • High yield 

  • Thermostable, up to 50°C, during first strand synthesis 

  • High processivity, generating cDNA up to 8 kb 

  • Reduced RNase H ribonuclease activity 


  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.


-20°C for 24 months 

Odoo - Sample 1 for three columns

High yield


Odoo - Sample 3 for three columns

High sensitivity

Odoo - Sample 2 for three columns

Thermostable, up to 50°C, during first strand synthesis






ExcelRT™ Reverse Transcriptase (200 U/μl) 

100 μl      

RNase Inhibitor (20 U/μl)

100 μl      

5X RT Buffer (DTT)

500 μl      

dNTPs Mix (10 mM each)

200 μl      

Oligo (dT)20 (50 μM)

100 μl      

Random Hexamers (100 μM)

100 μl      

DEPC-Treated H2O

  1 ml x 2

Storage Buffer

Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol

RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol

5X RT buffer (DTT)

250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2 and 50 mM DTT


-20°C for 24 months 


In silico identification and in vitro evaluation of MRPS30-DT lncRNA and MRPS30 gene expression in breast cancer

Nooshafarin Shirani, Roohallah Mahdi-Esferizi, Reza Eshraghi Samani, Shahram Tahmasebian, Hajar Yaghoobi

Cancer Rep (Hoboken). 2024 Jun;7(6):e2114. doi: 10.1002/cnr2.2114.

PMCID: PMC11182701

Effect of long-term inorganic nitrate administration on myocardial ischemia-reperfusion injury in ovariectomized rats

Sajad Jeddi, Nasibeh Yousefzadeh, Maryam Zarkesh, Khosrow Kashfi, Asghar Ghasemi

Front Pharmacol. 2024 Mar 27:15:1369379. doi: 10.3389/fphar.2024.1369379. eCollection 2024.

PMCID: PMC11004245

Beta arrestin‐related signalling axes are influenced by dexamethasone and metformin in vascular smooth muscle cells cultured in high glucose condition

Ali Akbar Soleimani, Nafiseh Shokri, Mohammad Elahimanesh, Payam Mohammadi, Najmeh Parvaz, Masoomeh Bakhshandeh, Mohammad Najafi

Endocrinol Diabetes Metab. 2024 Jan; 7(1): e465. Published online 2023 Dec 15. doi: 10.1002/edm2.465

PMCID: PMC10782052

In Vitro Targeted Delivery of Simvastatin and Niacin to Macrophages Using Mannan-Grafted Magnetite Nanoparticles

Banafsheh Rastegari, Atefe Ghamar Talepoor, Shahdad Khosropanah, Mehrnoosh Doroudchi

ACS Omega. 2024 Jan 9; 9(1): 658–674. Published online 2023 Dec 18. doi: 10.1021/acsomega.3c06389

PMCID: PMC10785661

Effect of drought stress on natural rubber biosynthesis and quality in Taraxacum kok-saghyz roots

Seyed Shahab Hedayat Mofidi, Mohammad Reza Naghavi, Manijeh Sabokdast, Parisa Jariani, Meisam Zargar, Katrina Cornish

PLoS One. 2024; 19(1): e0295694. Published online 2024 Jan 22. doi: 10.1371/journal.pone.0295694

PMCID: PMC10802950

Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis

Maryam Abtin, Nahid Nafisi, Asghar Hosseinzadeh, Sepideh Kadkhoda, Ramesh Omranipour, Leyla Sahebi, Masoumeh Razipour, Soudeh Ghafouri-Fard, Abbas Shakoori

Noncoding RNA Res. 2024 Jun; 9(2): 367–375. Published online 2024 Jan 26. doi: 10.1016/j.ncrna.2024.01.016

PMCID: PMC10950563

Assessment of expression of calcium signaling related lncRNAs in epilepsy

Mohammad Taheri, Ashkan Pourtavakoli, Solat Eslami, Soudeh Ghafouri-Fard, Arezou Sayad         

Sci Rep. 2023; 13: 17993. Published online 2023 Oct 21. doi: 10.1038/s41598-023-45341-7

PMCID: PMC10590428

Long-term inorganic nitrate administration protects against myocardial ischemia-reperfusion injury in female rats

Younes Yassaghi, Sajad Jeddi, Nasibeh Yousefzadeh, Khosrow Kashfi, Asghar Ghasemi    BMC Cardiovasc Disord. 2023 Aug 21;23(1):411. doi: 10.1186/s12872-023-03425-2.

PMCID: PMC10441752

Approaches to determine the efficiency of novel 3-phytase from Klebsiella pneumoniae and commercial phytase in broilers from 1 to 14 d of age

Mohammad Houshyar, Ali Asghar Saki, Mohammad Yousef Alikhani, Michael Richard Bedford, Meysam Soleimani, Farideh Kamarehei

Poult Sci. 2023 Aug 9;102(11):103014. doi:10.1016/j.psj.2023.103014. Online ahead of print.

PMCID: PMC10494260

Combined dandelion extract and all-trans retinoic acid induces cytotoxicity in human breast cancer cells

Hamed Rezaie, Reza Alipanah-Moghadam, Farhad Jeddi, Cain C T Clark, Vahideh Aghamohammadi, Ali Nemati     

Sci Rep. 2023 Sep 12;13(1):15074. doi: 10.1038/s41598-023-42177-z.

PMCID: PMC10497591

Altered sialin mRNA gene expression in type 2 diabetic male Wistar rats: implications for nitric oxide deficiency

Nasibeh Yousefzadeh, Sajad Jeddi, Maryam Zarkesh, Khosrow Kashfi , Asghar Ghasemi.  Sci Rep. 2023 Mar 10;13(1):4013. doi: 10.1038/s41598-023-31240-4.

PMCID: PMC10006425

MicroRNA-125b as a valuable predictive marker for outcome after autologous hematopoietic stem cell transplantation

Mohammad Rafiee, Fatemeh Amiri, Mohammad Hossein Mohammadi, Abbas Hajifathali.  BMC Cancer. 2023 Mar 3;23(1):202. doi: 10.1186/s12885-023-10665-0.

PMCID: PMC9983186

Quantification of the Chemical Chaperone 4-Phenylbutyric Acid (4-PBA) in Cell Culture Media via LC-HRMS: Applications in Fields of Neurodegeneration and Cancer

Salvatore Villani, Giulia Dematteis, Laura Tapella, Mara Gagliardi, Dmitry Lim, Marco Corazzari, Silvio Aprile and Erika Del Grosso

Pharmaceuticals (Basel). 2023 Feb; 16(2): 298. Published online 2023 Feb 14. doi: 10.3390/ph16020298

PMCID: PMC9960049

Heavy metal analysis in of indoor and outdoor dust extracts and cytotoxicity evaluation and inflammation factors on lung, gastric and skin cell lines  

Abolfazl Naimabadi, Ahmad Ghasemi, Mahnaz Mohtashami, Jafar Saeidi, Mehdi Bakaeian, Aliakbar Haddad Mashadrizeh, Mohsen Azimi-Nezhad, Ali Akbar Mohammadia

Heliyon. 2022 Dec; 8(12): e12414. Published online 2022 Dec 19. doi: 10.1016/j.heliyon.2022.e12414

PMCID: PMC9803783

Gene expression profile of placentomes and clinical parameters in the cows with retained placenta 

Mehdi Moradi, Mahdi Zhandi, Mohsen Sharafi, Arvand Akbari, Mohammad Jafari Atrabi, and Mehdi Totonchi

BMC Genomics. 2022; 23: 760. Published online 2022 Nov 21. doi: 10.1186/ s12864-022-08989-5

PMCID: PMC9677913

Assessment of Expression of Regulatory T Cell Differentiation Genes in Autism Spectrum Disorder

Mohammadarian Akbari, Reyhane Eghtedarian, Bashdar Mahmud Hussen, Solat Eslami, Mohammad Taheri, Seyedeh Morvarid Neishabouri, Soudeh Ghafouri-Fard, Front Mol Neurosci. 2022 Jul 4;15:939224. doi: 10.3389/fnmol.2022.939224. eCollection 2022.

PMCID: PMC9289514

Anti-inflammatory function of apolipoprotein B-depleted plasma is impaired in non-alcoholic fatty liver disease

Negar Sarmadi, Hossein Poustchi, Fatemeh Ali Yari, Amir Reza Radmard, Sara Karami, Abbas Pakdel, Parisa Shabani, Ali Khaleghian, PLoS One. 2022 Apr 12;17(4):e0266227. doi: 10.1371/journal.pone.0266227. eCollection 2022.

PMCID: PMC9004768

Expression analysis of IFNAR1 and TYK2 transcripts in COVID-19 patients

Mohammadarian Akbari, Mehdi Akhavan-Bahabadi, Navid Shafigh, Afshin Taheriazam, Bashdar Mahmud Hussen, Arezou Sayad, Mohadeseh Fathi, Mohammad Taheri, Soudeh Ghafouri-Fard, Mohammad Fathi, Cytokine. 2022 May;153:155849. doi: 10.1016/j.cyto.2022.155849. Epub 2022 Mar 4.

PMCID: PMC8894869

Focal adhesion kinase-related pathways may be suppressed by metformin in vascular smooth muscle cells in high glucose conditions

Ali Akbar Soleimani, Ghasem Ghasmpour, Asghar Mohammadi, Masoomeh Gholizadeh, Borhan Rahimi Abkenar, Mohammad Najafi, Endocrinol Diabetes Metab. 2022 May 28;e351. doi: 10.1002/edm2.351.

PMCID: PMC9258994

Direct RNA Nanopore Sequencing of SARS-CoV-2 Extracted from Critical Material from Swabs

Davide Vacca, Antonino Fiannaca, Fabio Tramuto, Valeria Cancila, Laura La Paglia, Walter Mazzucco, Alessandro Gulino, Massimo La Rosa, Carmelo Massimo Maida, Gaia Morello, Beatrice Belmonte, Alessandra Casuccio, Rosario Maugeri, Gerardo Iacopino, Carmela Rita Balistreri, Francesco Vitale, Claudio Tripodo, Alfonso Urso, Life (Basel). 2022 Jan 4;12(1):69. doi: 10.3390/life12010069.

PMCID: PMC8778588

Lycopene improves testicular damage and sperm quality in experimentally induced varicocele: Relationship with apoptosis, hypoxia, and hyperthermia

Atefeh Babaei, Reza Asadpour, Kamran Mansouri, Adel Sabrivand, Siamak Kazemi-Darabadi, Food Science & Nutrition March 2022. doi:10.1002/fsn3.2762 

PMCID: PMC9094497

Up-regulation of FOXN3-AS1 in invasive ductal carcinoma of breast cancer patients 

Samira Molaei Ramshe 1, Hamid Ghaedi 1, Mir Davood Omrani 2, Lobat Geranpayeh 3, Behnam Alipour 4, Soudeh Ghafouri-Fard, Heliyon

. 2021 Oct 14;7(10):e08179. doi: 10.1016/j.heliyon.2021.e08179. eCollection 2021 Oct.

PMCID: PMC8526775

Expression Analysis of Long Non-Coding RNAs Related With FOXM1, GATA3, FOXA1 and ESR1 in Breast Tissues

Bita Hassani,  Mohammad Taheri,  Yazdan Asgari,  Ali Zekri,  Ali Sattari,  Soudeh Ghafouri-Fard, * and Farkhondeh Pouresmaeili , Front Oncol. 2021; 11: 671418. Published online 2021 May 19. doi: 10.3389/fonc.2021.671418

PMCID: PMC8171254

Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis via Suppression of C-C Chemokine Receptor Type 7 Expression

Pelin Kaya, Sang R. Lee, Young Ho Lee, Sun Woo Kwon, Hyun Yang, Hye Won Lee, and Eui-Ju Hong1, Nutrients. 2019 Feb; 11(2): 410. Published online 2019 Feb 15. doi: 10.3390/nu11020410

PMCID: PMC6412318

Odoo - Sample 1 for three columns

[RP1400] ExcelRT™ Reverse Transcription Kit II

  • Contains all components for reverse transcription

  • High yield 

  • Thermostable, up to 50°C 
    Reduced RNase H ribonuclease activity 

  • Suitable for real-time PCR 

Odoo - Sample 3 for three columns

[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR